The Importance of Characterizing your Critical Reagents - Trastuzumab as a Case Study

• Optimization of Critical Reagents labeling is important for proper Anti-Drug Antibodies (ADA) assessment;
• Biotin and Sulfo-tag conjugated trastuzumab have discrete optimal molar incorporation ratios;
• Free unlabeled biotin affects assay performance while free sulfo-tag does not
• Freeze / thaw cycles negatively impact the Sulfo-tagged trastuzumab performance.

Click Here to read the full Case Study by Agostinho G. Rocha, PhD*; Anthony Mancino; Matthew Sciscione; Katelyn Krynski; Jason Vergara; Christopher Beaver, PhD Syneos Health, Princeton, NJ, USA